Synaptic reconstruction of all excitatory and inhibitory inputs made onto a single cortical pyramidal neuron labeled with sparse in utero electroporation. (Daniel Iascone)
Workflow for excitatory and inhibitory synaptic reconstruction across whole neurons with Vaa3D Synapse Detector program developed in collaboration with the Allen Institute for Brain Science. (Daniel Iascone)
Pyramidal neurons in the CA1 region of the hippocampus labeled by in utero electroporation, visualized by large a volume confocal scan. (Unpublished, Heike Blockus)
A cortical layer 2/3 neuron at 21 days in vitro labeled by ex utero electroporation with fluorescent marker for spine visualization. (Unpublished, Heike Blockus)
Callosal axon branching (green, contralateral side) labeled by in utero cortical electroporation at E15, examined at P21 (from Julien Courchet). Red counterstaining (Cux1) labels layer 2-4.
Courchet J.*, Lewis T. Jr *, Aizawa S. and Polleux F. (2013) Terminal axon branching is regulated by the LKB1-NUAK1 kinase pathway via presynaptic mitochondrial capture.
Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP+ pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ). Red channel: CTIP2 (layer 5 and 6a marker).
Image obtained by Randal Hand, see the following article for more details:
Hand R., Polleux F. (2011)
Neurogenin2 regulates the initial axon guidance of cortical pyramidal neurons projecting medially to the corpus callosum.
Neural Development Aug 24; 6:30.
Synaptic reconstruction of all excitatory inputs made onto a single cortical pyramidal neuron labeled with sparse in utero electroporation (Unpublished, Dan Iascone)
Isolated excitatory spines annotated from a single cortical pyramidal neuron with Vaa3D Synapse Detector program developed in collaboration with the Allen Institute for Brain Science (Unpublished, Dan Iascone)