ctrl 6529-4

Callosal axon branching (green, contralateral side) labeled by in utero cortical electroporation at E15, examined at P21 (from Julien Courchet). Red counterstaining (Cux1) labels layer 2-4.

Modified from:

Courchet J.*, Lewis T. Jr *, Aizawa S. and Polleux F. (2013) Terminal axon branching is regulated by the LKB1-NUAK1 kinase pathway via presynaptic mitochondrial capture.
Cell 153:1510-1525.

*Contributed equally to this work.
E14 electro+CTIP2

Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP+ pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ). Red channel: CTIP2 (layer 5 and 6a marker). 

Image obtained by Randal Hand, see the following article for more details:

Hand R., Polleux F. (2011)
Neurogenin2 regulates the initial axon guidance of cortical pyramidal neurons projecting medially to the corpus callosum.
Neural Development Aug 24; 6:30.


19 days in vitro layer 2/3 cortical neuron labeled via sparse ex utero electroporation (E15.5) with mitoDSRED (red, mitochondria) and VGLUT1-Venus (green, presynaptic sites). (Unpublished, Tommy Lewis)
Cortical neuronal culture in which presynaptic inputs (red, tdTomato) to a single cortical neuron (green) were visualized using rabies virus monosynaptic tracing. (Unpublished, Ewoud Schmidt)
Presynaptic neurons (red, tdTomato) traced in vivo from a sparse population of in utero electroporated layer 2/3 cortical neurons (green, nuclear GFP) using rabies virus monosynaptic tracing. (Unpublished, Ewoud Schmidt)
A mature cortical neuron in culture expressing SRGAP2 (green), tdTomato (red), and the inhibitory synapse marker, gephyrin (blue). SRGAP2 is enriched in dendritic spines. (Unpublished, Justine Kupferman)
Electron microscopy image of a layer 2/3 cortical neuron highlighted by DAB staining.
(Unpublished, Yusuke Hirabayashi)
The ER-mitochondria interaction sites labeled by split-GFP (Red:ER, Blue:Mitochondria) visualized using super-resolution microscopy (SIM).
(Unpublished, Yusuke Hirabayashi)
Dendritic spines (yellow) reconstructed from the apical dendrite of an L2/3 pyramidal neuron (red; tdTomato) labeled by in uteroelectroporation at E15 and examined at P21 (Unpublished, Dan Iascone)